The resulting supernatant was discarded and the mitochondrial pellet re-suspended in mitochondrial storage buffer for use in subsequent assays
The resulting supernatant was discarded and the mitochondrial pellet re-suspended in mitochondrial storage buffer for use in subsequent assays. Immunoblot analyses For whole cell westerns, cells were lysed in RIPA buffer [50 mM Tris, pH 7.4, 1% Igepal, 0.25% sodium deoxycholate, 150 mM NaCl, 1 mM EDTA, and a proteinase inhibitor cocktail (Roche Molecular Biochemicals)]. […]
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