Measurement of Foxp3 gene expression by qRT-PCR RNA of digested lung cells was isolated using the Absolutely RNA Mini Prep kit (Stratagene, La Jolla, CA), according to the manufacturers protocol
Measurement of Foxp3 gene expression by qRT-PCR RNA of digested lung cells was isolated using the Absolutely RNA Mini Prep kit (Stratagene, La Jolla, CA), according to the manufacturers protocol. pulmonary CD4+ T cells, expressing Foxp3-protein or FR4 remained stable. Therefore, the inhibition by V1+ T cells might not be targeting Foxp3+ Treg but rather […]
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