Transwell chambers were incubated for 24?h

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Transwell chambers were incubated for 24?h. epidermal growth factor (EGF; Thermo Fisher Scientific, USA), and 10?ng/mL basic fibroblast growth factor (bFGF; PeproTech, USA). GBM and NHA cells were cultured in Dulbeccos altered Eagles medium (DMEM; Life Technologies-Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS; Life Technologies-Thermo Fisher Scientific, USA) and maintained at […]