The purified fractions of rSEB was dialyzed against 1X PBS for 72h at 4C with 3 buffer changes and confirmed by SDS-PAGE and Western-blotting analysis using anti-SEB polysera (Sigma, India)
The purified fractions of rSEB was dialyzed against 1X PBS for 72h at 4C with 3 buffer changes and confirmed by SDS-PAGE and Western-blotting analysis using anti-SEB polysera (Sigma, India). == Selection of ssDNA aptamers against SEB by SELEX method == The ssDNA aptamer library used in the present study consists of a central region […]
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