The purified fractions of rSEB was dialyzed against 1X PBS for 72h at 4C with 3 buffer changes and confirmed by SDS-PAGE and Western-blotting analysis using anti-SEB polysera (Sigma, India)

jamha May 4, 2025 0 Comments

The purified fractions of rSEB was dialyzed against 1X PBS for 72h at 4C with 3 buffer changes and confirmed by SDS-PAGE and Western-blotting analysis using anti-SEB polysera (Sigma, India). == Selection of ssDNA aptamers against SEB by SELEX method == The ssDNA aptamer library used in the present study consists of a central region […]

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Significant studies and work have increasingly proven that probiotics provide methods to a well balanced gut microbiota in poultry, maintaining health status in broilers, preserving gut condition and increasing immune system aswell as enhancing nutritional absorption, which are needed and essential to promote growth of broilers

jamha October 8, 2024 0 Comments

Significant studies and work have increasingly proven that probiotics provide methods to a well balanced gut microbiota in poultry, maintaining health status in broilers, preserving gut condition and increasing immune system aswell as enhancing nutritional absorption, which are needed and essential to promote growth of broilers. Acknowledgments This work was supported Teroka Jaya Farm (Penang, […]

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HLA-DRB1 genotyping Dried blood from individual samples were reconstituted in 200 l water, with DNA extracted from individual blood and control saliva samples using QIAamp? blood kits (Qiagen, Hilden, Germany)

jamha September 26, 2024 0 Comments

HLA-DRB1 genotyping Dried blood from individual samples were reconstituted in 200 l water, with DNA extracted from individual blood and control saliva samples using QIAamp? blood kits (Qiagen, Hilden, Germany). locus association with (p 2.4 10?14). Five alleles exhibited significant T1D association: three alleles (experienced the strongest association (odds ratio=5.04, p=1.710?10). Conclusions: Young Sudanese individuals […]

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The effect of IC50 value of APAU on the recombinant rat sEH enzyme with a fluorescent substrate is 6nM (Hwang et al

jamha October 31, 2022 0 Comments

The effect of IC50 value of APAU on the recombinant rat sEH enzyme with a fluorescent substrate is 6nM (Hwang et al., 2007). acids are endogenous lipid metabolites with important roles in cellular signaling which is underscored by their tight regulation (Bernstrom et al., 1992; Spector and Norris, 2007). These epoxy-metabolites are formed by cytochrome […]

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Secondary antibodies were Amersham ECL donkey anti-rabbit or sheep anti-mouse horseradish peroxidase (HRP)-linked IgG antibody (both GE Healthcare UK Limited, Little Chalfont, UK)

jamha January 10, 2022 0 Comments

Secondary antibodies were Amersham ECL donkey anti-rabbit or sheep anti-mouse horseradish peroxidase (HRP)-linked IgG antibody (both GE Healthcare UK Limited, Little Chalfont, UK). growth medium only or induced to osteogenically differentiate by the addition of health supplements (-glycerophosphate, ascorbic acid, dexamethasone, and 1,25-dihydroxyvitamin D3). The activation of and requirement for PI3K/Akt signaling in MSC osteogenesis […]

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In particular, p53 mainly mediates prosenescence signals that are derived via oncogene activation, telomere dysfunction, DNA damage, and reactive oxygen species [9]

jamha October 1, 2021 0 Comments

In particular, p53 mainly mediates prosenescence signals that are derived via oncogene activation, telomere dysfunction, DNA damage, and reactive oxygen species [9]. dickkopf-1 (an antagonist of the Wnt coreceptor) and -catenin siRNA transfection promotes senescence in MSCs. Interestingly, the magnitude of the response to enhanced Wnt3a/-catenin signaling appears to depend on the senescent state during […]

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